FIG. 3.

Reovirus-induced host shutoff in PKR KO MEFs transfected with human PKR. (A) PKR expression in cell extracts from iPKR KO+BAC-huPKR cells, PKR wt MEFs, and PKR KO MEFs (lanes 1 to 3, respectively) was examined by immunoblot analysis. (B) iPKR KO+BAC-huPKR cells were mock infected or infected in triplicate with the indicated reovirus strains at an MOI of 80 PFU/cell. Levels of cellular translation were analyzed at 20 h p.i. as described in the legend to Fig. 2.