FIG. 4.
Effect of nonphosphorylatable, constitutively active eIF2α on reovirus-induced host shutoff. (A and B) wt eIF2α (A) or S51A eIF2α (B) MEFs were mock infected or infected in triplicate with the indicated reovirus strains at an MOI of 80 PFU/cell. Cells were metabolically labeled at 20 h p.i. with [35S]methionine-cysteine for 2 h. Levels of cellular translation were determined as described in the legend to Fig. 2. (C) Graph depicting results from three separate experiments (Expt). The black bars represent the level of cellular translation in wt eIF2α MEFs; the grey bars represent protein synthesis in S51A eIF2α MEFs. ND, not determined.