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. 2017 May 15;114(22):E4492–E4500. doi: 10.1073/pnas.1700721114

Fig. 2.

Fig. 2.

Characterization of activity of compound 1 against DNA gyrase. (A) Inhibition of E. coli DNA gyrase supercoiling activity by compound 1. Relaxed (Rel) pBR322 DNA was incubated with gyrase and different concentrations of compound and separated by agarose gel electrophoresis. (B) Stabilization of the cleavage complex with E. coli DNA gyrase by compound 1. Supercoiled pBR322 DNA was incubated with gyrase and different concentrations of compound 1. Cleaved DNA complexes were trapped with SDS, followed by proteinase K digestion, and DNA was separated by agarose gel electrophoresis. L, linearized DNA; N, nicked DNA; SC, supercoiled DNA.