Expression and methylation levels of representative cluster 1 and cluster 2 genes in fruits of the WT and sldml2 mutants. RT-qPCR measurements to validate the expression of two cluster 1 genes, SlPSY1 and SlACS4 (A), and two cluster 2 genes, SlCAP10B and SlRBCS-2A (B), in WT and sldml2-1 mutant fruits at 25 dpa and 46 dpa. EF1α was used as the internal control. The DNA methylation levels of promoter regions of these four genes are shown with screenshots of Integrative Genome Browser (IGB) display of whole-genome bisulfite sequencing data, where each vertical bar represents an mC and the height of the bar indicates methylation level. Error bars indicate SD, n = 3 (*P value < 0.01, two-tailed t test).