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Characterization of gp78RING-Ube2g2 chimera. Time courses comparing the rates of ubiquitin polymerization by 0.1 µM Ube2g2 alone or in the presence of 20 µM gp78RING (A) or when Ube2g2 is fused directly to gp78RING (B; assayed at 0.1 µM). The chimera is more than sixfold faster than the unfused proteins.
