Table S1.

Proteins used in this study

RDB no.	Plasmid name	Vector	Expression	Purification	Comments	Source
3019	Ube1	pET28b	BL21, 0.5 mM IPTG, 16 °C for 16 h	Ni-NTA, S200	—	98
2804	Cdc34	pGEX-6P-1	BL21, 0.4 mM IPTG, 25 °C for 16 h	Glutathione–Sepharose, S200	—	76
3166	gp78-Ube2g2	pET28a	BL21, 0.4 mM IPTG, 25 °C for 16 h	Ni-NTA, S75, TEV cleavage, MonoQ	—	This study
2805	His-Ub	pET15b	BL21, 0.4 mM IPTG, 16 °C for 16 h	Ni-NTA, thrombin cleavage, S75	—	76
3348	His-Ub-K48R	pET15b	BL21, 0.4 mM IPTG, 16 °C for 16 h	Ni-NTA, S75	—	This study
3006	His-Ub-G76V-GFP	pET28a	BL21, 0.4 mM IPTG, 37 °C for 4 h	Ni-NTA, S200	—	This study
3344	His-Ub2-G76V-GFP	pET28a	BL21, 0.4 mM IPTG, 37 °C for 4 h	Ni-NTA, S200	—	This study
3345	His-Ub3-G76V-GFP	pET28a	BL21, 0.4 mM IPTG, 37 °C for 4 h	Ni-NTA, S200	—	This study
3219	p97-His	pET24b	Rosetta, 0.1 mM IPTG, 25 °C for 16 h	Ni-NTA, S6	—	99
3346	p97-His E305Q	pET24b	Rosetta, 0.1 mM IPTG, 25 °C for 16 h	Ni-NTA, S6	—	This study
3347	p97-His E578Q	pET24b	Rosetta, 0.1 mM IPTG, 25 °C for 16 h	Ni-NTA, S6	—	This study
3352	p97-His A232E	pET24b	Rosetta, 0.1 mM IPTG, 25 °C for 16 h	Ni-NTA, S6	—	This study
2686	NPLOC4	pCOLA-Duet	BL21, 0.4 mM IPTG, 16 °C for 16 h	Ni-NTA, S200	Copurified with UFD1L	100
2796	His-UFD1LL	pET28a	BL21, 0.4 mM IPTG, 16 °C for 16 h	Ni-NTA, S200	Copurified with NPLOC4	62
2886	His-NSFL1C	pTrcHis	TOP10, 0.4 mM IPTG, 37 °C for 4 h	Ni-NTA, S200	—	39
2598	GST-FLAG-UBXN7	pGEX-4T-2-TEV	Rosetta, 0.4 mM IPGT, 37 °C for 4 h	Glutathione Sepharose, TEV cleavage, S200	—	43
3218	GroEL D87K	T7	TOP10, 0.4 mM IPTG, 37 °C for 4 h	HiTrap Q FF, S6, Affigel Blue	—	78