Table S1.

Inward and outward CatSper currents

Figure no.	Experimental condition	At –80 mV, pA/pF	At +80 mV, pA/pF	I/Imax at –80 mV	n, number of cells	Number of donors
Fig. 1A	DVF	−49.6 ± 19.6	146.2 ± 10.0		7	3
	1 μM P4	−322.9 ± 38.2	430.2 ± 41.3		7	3
Fig. 1B	DVF	−30.6 ± 7.4	195.3 ± 25.8		8	2
	1 μM T	−29.0 ± 4.7	136.7 ± 22.7		8	2
	1 μM T + 1 μM P4	−44.5 ± 10.6	135.4 ± 27.6		8	2
Fig. 1C	DVF	−43.0 ± 4.4	146.2 ± 10.0		4	2
	1 μM E2	−55.9 ± 7.1	170.1 ± 4.9		4	2
	1 μM E2 + 1 μM P4	−154.9 ± 14.2	250.4 ± 17.4		4	2
Fig. 1D	DVF	−37.2 ± 6.6	222.7 ± 27.0		5	2
	1 μM HC	−39.9 ± 9.7	192.5 ± 14.8		5	2
	1 μM HC + 1 μM P4	−153.3 ± 21.2	347.5 ± 16.2		5	2
Fig. 1E	DVF	−42.8 ± 7.8	171.5 ± 15.6		13	3
	1 μM PregS	−155.5 ± 16.8	267.7 ± 21.6		13	3
Fig. 1F	0.01 μM T + 1 μM P4			1.03 ± 0.05	5	2
	0.1 μM T + 1 μM P4			0.87 ± 0.1	7	2
	0.5 μM T + 1 μM P4			0.48 ± 0.04	3	2
	1 μM T + 1 μM P4			0.11 ± 0.01	7	2
Fig. 1G	0.00001 μM E2 + 1 μM P4			0.88 ± 0.07	3	2
	0.001 μM E2 + 1 μM P4			0.80 ± 0.09	5	2
	0.1 μM E2 + 1 μM P4			0.61 ± 0.06	5	2
	1 μM E2 + 1 μM P4			0.48 ± 0.04	4	2
Fig. 1H	0.001 μM HC + 1 μM P4			1.00 ± 0.06	4	2
	0.1 μM HC + 1 μM P4			0.47 ± 0.07	3	2
	1 μM HC + 1 μM P4			0.36 ± 0.05	5	2
	10 μM HC + 1 μM P4			0.10 ± 0.01	3	2

All values are presented as mean ± SEM.