Table S2.

Inward and outward CatSper currents

Figure no.	Experimental condition	At –80 mV, pA/pF	At +80 mV, pA/pF	I/Imax at –80 mV	n, number of cells	Number of donors
Fig. 2B	DVF	−71.4 ± 15.7	308.9 ± 33.7		4	2
	1 μM PregS	−225.3 ± 32.2	423.1 ± 44.8		4	2
	1 μM PregS + 1 μM NNC	−34.9 ± 1.5	43.7 ± 1.9		4	2
Fig. 2D	0.001 μM PregS			0.21 ± 0.03	5	3
	0.01 μM PregS			0.39 ± 0.10	4	3
	0.1 μM PregS			0.76 ± 0.07	4	3
	1 μM PregS			1.00 ± 0.00	13	3
	5 μM PregS*			0.90 ± 0.02	13	3
	0.1 μM PregS + 1 μM T			0.35 ± 0.02	3	2
	0.5 μM PregS + 1 μM T			0.70 ± 0.05	3	2
	1 μM PregS + 1 μM T			1.00 ± 0.07	4	2
Fig. 2F	DVF	−42.8 ± 7.8	216.6 ± 7.0		16	3
	2 μM MAFP	−33.5 ± 5.9	85.0 ± 35.5		3	2
	2 μM MAFP + 1 μM PregS	−54.1 ± 14.7	120.7 ± 57.4		3	2
	1 μM PregS	−155.5 ± 16.8	267.7 ± 21.6		13	3

All values are presented as mean ± SEM.

^*This data point was excluded from the fit due to a nonspecific effect of this high lipid concentration on cell plasma membrane.