Fig. 2.

LLG1 is required for basal defenses. (A) Growth of G. cichoracearum on Col-0, llg1-2, llg1-3, and a representative LLG1 llg1-3 complementation line. Four-week-old plants were inoculated with G. cichoracearum. The number of conidiophores per colony was counted at 5 dpi. Error bars represent SD from three independent experiments (n = 25). (B) Growth of H. arabidopsidis (H.a.) Noco2 on wild-type and mutant plants. Two-week-old plants were inoculated with H. arabidopsidis Noco2 spores at a concentration of 5 × 10⁴ spores per milliliter. Pathogen growth was scored at 7 dpi. Error bars represent SD from three independent experiments (n = 4). (C) Disease-resistance assay using Pto DC3000. Four-week-old plants were spray-inoculated with Pto DC3000 bacteria at 10⁸ cfu/mL. Bacterial growth was scored at 4 dpi. Error bars represent SD from three independent experiments (n = 4). (D) The accumulation of PR1 transcripts was analyzed by quantitative real-time RT-PCR after infection with G. cichoracearum at 0 and 3 dpi. ACTIN2 was used as an internal control. Error bars represent SD from three independent experiments (n = 3). (E) Free SA was extracted from uninfected and infected (3 dpi) leaves of 4-wk-old plants with G. cichoracearum. Error bars represent SD from three independent experiments (n = 3). Lowercase letters indicate statistically significant differences (P < 0.05, nested ANOVA).