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. 2017 May 15;114(22):5749–5754. doi: 10.1073/pnas.1614468114

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Fig. 5. — LLG1 is required for flg22-induced BIK1 phosphorylation. (A) Co-IP of FLS2-HA and BAK1 in Arabidopsis protoplasts expressing FLS2-HA before (−) or 10 min (+) after treatment with 1 μM flg22. FLS2-HA and BAK1 were detected by immunoblot analysis with anti-HA or anti-BAK1 antibody, respectively, after IP with anti-HA antibody. (B) Immunoblot analysis of BIK1-HA proteins in Arabidopsis protoplasts expressing BIK1-HA before (−) or 10 min (+) after treatment with 1 μM flg22. The proteins were detected by anti-HA antibody. PPase represents protein phosphatase. BAK1 was used as the loading control. All experiments were repeated at least three times with similar results.