Fig. 6.

LLG1 is required for accumulation and flg22-induced degradation of FLS2. (A) Confocal micrographs of 4-wk-old fls2, llg1-2, and llg1-3 transgenic plants expressing FLS2–YFP and showing leaf epidermal cells. Micrographs are maximum projections of 8–10 confocal optical sections taken at a 1-μm z-distance using the same microscope settings. (Scale bars, 5 μm.) Similar results were observed in at least three independent experiments. (B) Four-week-old plants were treated with 0 µM or 10 µM flg22 for 50 min. Each lane was loaded with 10 µg of total protein. FLS2–YFP was detected with anti-GFP antibody, and the molecular size for the band was indicated. H⁺-ATPase was used as plasma membrane protein marker. The experiment was repeated three times with similar results. FLS2–YFP protein levels were quantified with ImageJ. Error bars represent SD from three independent experiments (n = 3). Lowercase letters indicate statistically significant differences (P < 0.05, nested ANOVA). C, cytosol protein; PM, plasma membrane protein; T, total protein.