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. 2017 May 15;114(22):5749–5754. doi: 10.1073/pnas.1614468114

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Fig. S6. — LLG1 did not interact with EDR1. (A) LLG1 did not interact with EDR1 in yeast two-hybrid assay. EDR1 and MKK5 were used as a positive control. Yeast cells containing the indicated plasmids were spotted onto the SD/-Trp/-Leu or SD/-Trp/-Leu/-His/-Ade medium as indicated. (B) LLG1 did not interact with EDR1 in Co-IP assays in N. benthamiana. EDR1-FLAG and MKK5-HA were used as a positive control. Agrobacterium containing the indicated plasmids were infiltrated into N. benthamiana, and proteins were extracted and subjected to Co-IP analysis, as indicated. All experiments were repeated three times with similar results.