Fig. S7.

The YFP–LLG1 fusion protein is functional. (A) The 35S:YFP–LLG1 construct complemented the llg1-3 phenotype. Four-week-old plants were inoculated with G. cichoracearum and the number of conidiophores per colony was counted at 5 dpi. An asterisk represent significant differences (P < 0.01) using Student’s t test. (B) YFP–LLG1 is properly expressed in 35S:YFP–LLG1 (in llg1-3 background) transgenic plants. The immunoblot analysis was performed using anti-GFP antibody in 2-wk-old plants. Ponceau S staining of the large subunit of Rubisco is shown as a loading control. (C) Confocal micrographs of YFP–LLG1 fluorescence in leaf epidermis and root cells of 35S:YFP–LLG1 transgenic plants. (Scale bar, 20 μm.) (D) Confocal micrographs of root cells of 35S:YFP–LLG1 transgenic plants stained with FM4-64. (Scale bar, 10 μm.) All experiments were repeated three times with similar results.