Fig. 1.

Genome-wide screen identifies genes that affect DNA replication fidelity in S. cerevisiae. (A) Strategy used to cross the nonessential gene deletion collection with active-site DNA polymerase mutants. (B) To screen for mutator phenotypes in 96-well format, strains were spotted on YPD, grown, and replica plated on mutator reporter plates. Increased number of colonies is indicative of a potential mutator phenotype. On the right side (zoom-in), msh6Δ results in increased frameshifts (lysine−) and CAN1 mutations (+canavanine), whereas ubc13Δ increases CAN1 mutations, exclusively.