Fig. 3.

Inactivation of Ura7 or Gln3 results in DDR checkpoint activation. (A) Simplified diagram depicting DDR response in S. cerevisiae. (B) Whole-cell lysates of logarithmically growing cells were analyzed by Western blotting with Rad53 and RNR1-4 antibodies. WT cells treated with 200 mM hydroxyurea (HU) were used as control for activation of DDR. (C) DNA content profiles of the indicated strains. (D) Mutation rates in mutant strains in the presence or absence of DUN1. See also Table S3. (E) Mutation rates in the indicated strains grown in YPD media supplemented or not with 5 mM glutamine (Gln). Error bars represent the 95% CI, and numbers on top indicate the fold increase in the mutation rate over WT.