Table S3.

Mutation rate analysis for several mutants

	Mutation rate (fold increase)*
Relevant genotype	CanR	Lys+	Thr+
WT	7.2 [5.7–9.0] × 10−8 (1)	1.5 [0.8–2.2] × 10−8 (1)	2.1 [1.4–3.2] × 10−9 (1)
gln3Δ	1.0 [0.8–1.2] × 10−7 (1)	1.6 [1.1–3.7] × 10−8 (1)	2.4 [1.7–3.7] × 10−9 (1)
shm2Δ	1.2 [1.1–1.7] × 10−7 (2)	3.1 [1.2–5.0] × 10−8 (2)	2.6 [1.7–5.6] × 10−9 (1)
ura7Δ	1.0 [0.9–1.5] × 10−7 (1)	1.4 [1.0–2.5] × 10−8 (1)	1.9 [1.2–5.6] × 10−9 (1)
rrm3Δ	1.1 [0.8–1.5] × 10−7 (2)	2.4 [1.3–3.0] × 10−8 (2)	4.6 [2.6–7.9] × 10−9 (2)
exo1Δ	7.4 [6.3–9.8] × 10−7 (10)	1.4 [0.9–1.8] × 10−7 (10)	8.7 [6.1–15.0] × 10−9 (4)
exo1Δ gln3Δ	1.1 [0.8–1.4] × 10−5 (146)	1.2 [0.7–1.6] × 10−6 (83)	3.5 [2.7–5.0] × 10−7 (170)
exo1Δ shm2Δ	8.4 [7.1–10.1] × 10−7 (12)	3.5 [2.4–5.1] × 10−7 (24)	1.8 [1.1–2.5] × 10−8 (9)
exo1Δ ura7Δ	1.9 [0.8–2.4] × 10−5 (261)	1.3 [0.7–1.9] × 10−6 (89)	6.6 [4.9–8.3] × 10−7 (319)
exo1Δ rrm3Δ	6.3 [4.3–7.6] × 10−7 (9)	1.3 [1.0–1.8] × 10−7 (9)	2.5 [2.0–3.1] × 10−8 (12)
msh2Δ	5.4 [4.4–7.2] × 10−6 (75)	9.9 [8.1–10.8] × 10−5 (6,771)	6.3 [5.2–12.8] × 10−6 (3,053)
msh2Δ gln3Δ	1.3 [0.8–2.1] × 10−5 (177)	8.7 [6.9–14.9] × 10−5 (5,972)	4.5 [3.1–6.5] × 10−6 (2,149)
msh2Δ shm2Δ	7.4 [4.8–8.6] × 10−6 (104)	1.4 [1.1–2.1] × 10−4 (9,737)	6.1 [4.4–8.2] × 10−6 (2,918)
msh2Δ ura7Δ	3.5 [2.6–4.2] × 10−5 (492)	6.1 [4.7–8.8] × 10−5 (4,161)	5.7 [4.1–8.5] × 10−6 (2,738)
msh2Δ rrm3Δ	1.7 [1.2–2.6] × 10−5 (234)	1.1 [0.9–1.2] × 10−4 (7,198)	1.6 [1.1–2.4] × 10−5 (7,491)
msh3Δ	1.1 [0.8–1.2] × 10−7 (1)	2.5 [2.0–3.0] × 10−7 (17)	2.7 [2.0–4.2] × 10−8 (13)
msh3Δ gln3Δ	1.6 [1.1–2.6] × 10−7 (2)	1.9 [1.4–2.3] × 10−7 (13)	1.8 [1.5–1.9] × 10−8 (9)
msh3Δ shm2Δ	1.5 [1.3–2.9] × 10−7 (2)	1.2 [1.3–2.6] × 10−7 (12)	2.7 [1.7–3.7] × 10−8 (13)
msh3Δ ura7Δ	1.5 [1.3–2.0] × 10−7 (2)	1.2 [0.8–2.1] × 10−7 (8)	1.7 [1.1–3.0] × 10−8 (8)
msh3Δ rrm3Δ	2.6 [1.9–3.4] × 10−7 (4)	3.7 [3.5–4.3] × 10−7 (25)	6.1 [4.9–8.3] × 10−8 (30)
msh6Δ	9.6 [7.8–11.7] × 10−7 (13)	1.3 [0.9–1.6] × 10−6 (86)	1.3 [0.9–1.6] × 10−8 (6)
msh6Δ gln3Δ	2.4 [1.7–3.4] × 10−5 (334)	1.2 [0.7–4.0] × 10−6 (80)	1.0 [0.6–1.6] × 10−7 (48)
msh6Δ shm2Δ	2.1 [1.3–2.6] × 10−6 (30)	1.0 [0.9–1.3] × 10−6 (71)	3.5 [2.7–5.4] × 10−8 (17)
msh6Δ ura7Δ	3.8 [3.2–8.5] × 10−5 (524)	8.6 [6.6–20.6] × 10−7 (59)	9.2 [4.5–26.2] × 10−8 (44)
msh6Δ rrm3Δ	4.9 [3.6–7.3] × 10−6 (68)	9.1 [6.1–13.8] × 10−7 (62)	5.5 [3.9–6.8] × 10−8 (26)
dun1Δ	5.6 [4.2–9.1] × 10−8 (1)	2.1 [0.9–3.5] × 10−8 (1)	2.4 [1.7–5.2] × 10−9 (1)
pol1-L868M dun1Δ	9.6 [5.4–15.0] × 10−8 (1)	nd	nd
pol2-04 gln3Δ dun1Δ	8.6 [6.4–16.5] × 10−8 (1)	nd	nd
pol2-04 dun1Δ	9.4 [6.5–17.4] × 10−8 (1)	nd	nd
pol3-L612M gln3Δ dun1Δ	3.1 [1.8–4.0] × 10−6 (43)	nd	nd
exo1Δ ura7Δ dun1Δ	8.5 [5.4–11.3] × 10−7 (12)	2.6 [1.8–3.6] × 10–7 (18)	8.3 [4.7–10.7] × 10−9 (4)

Relates to Tables 1 and 2 and Fig. 3.

^*Median rates of inactivation of CAN1 gene (Can^R) and lys2-10A (Lys⁺) and hom3-10 (Thr⁺) reversion with 95% confidence interval in square brackets and fold increase relative to the WT in parentheses. “nd” indicates not determined.