Table S6.
CAN1 mutation spectra
| Mutations observed | WT | msh6Δ | msh6Δ gln3∆ | msh6Δ shm2∆ | msh6Δ ura7∆ |
| CanR clones sequenced | 91 | 110 | 94 | 95 | 110 |
| Mutations overall* | 92 (100) | 111 (100) | 96 (100) | 96 (100) | 110 (100) |
| Base substitutions | 69 (75) | 102 (92) | 95 (99) | 95 (99) | 109 (99) |
| A-T → G-C | 6 (7) | 9 (8) | 1 (1) | 1 (1) | 0 (0) |
| G-C → A-T | 18 (20) | 60 (54) | 92 (96) | 77 (80) | 104 (95) |
| G-C → T-A | 29 (32) | 27 (24) | 0 (0) | 14 (15) | 5 (5) |
| A-T → C-G | 3 (3) | 2 (2) | 0 (0) | 3 (3) | 0 (0) |
| A-T → T-A | 7 (8) | 1 (1) | 0 (0) | 0 (0) | 0 (0) |
| C-G → G-C | 6 (7) | 3 (3) | 2 (2) | 0 (0) | 0 (0) |
| Transitions | 24 (26) | 69 (62) | 93 (97) | 78 (81) | 104 (95) |
| Transversions | 45 (49) | 33 (30) | 2 (2) | 17 (18) | 5 (5) |
| One-base pair frameshifts | 15 (16) | 7 (6) | 1 (1) | 1 (1) | 1 (1) |
| Complex mutations† | 8 (9) | 2 (2) | 0 (0) | 0 (0) | 0 (0) |
Relates to Fig. 4. Mutation spectra analysis based on DNA sequencing of the CAN1 gene in independent CanR mutants, shown as the number of clones containing the indicated mutations, and in parentheses as the percentage relative to the total.
*
In few cases (about 1–2% of the sequenced clones), two simultaneous CAN1 mutations (more than 100 bp apart) were found. These mutations were included in the analysis and considered as independent mutational events.
†
Include multiple mutations within 10 nt, insertions or deletions of more than 1 nt, and duplication events.