Table 1.
Mutation rate analysis of the mutants identified in this screen in combination with DNA polymerase active-site mutant alleles
| Mutation rate (fold increase)* CanR | ||||
| Relevant genotype | WT | pol1-L868M | pol2-M644G | pol3-L612M |
| WT | 7.2 [5.7–9.0] × 10−8 (1) | 3.9 [3.3–4.9] × 10−7 (5) | 8.4 [7.3–10.6] × 10−7 (12) | 9.3 [7.7–11.6] × 10−7 (13) |
| exo1Δ | 7.4 [6.3–9.8] × 10−7 (10) | 5.7 [3.1–8.1] × 10−6 (80) | 1.9 [1.1–2.9] × 10−6 (26) | 6.5 [3.6–10.8] × 10−6 (91) |
| gln3Δ | 1.0 [0.8–1.2] × 10−7 (1) | 2.1 [1.4–4.5] × 10−5 (293) | 3.3 [2.6–6.0] × 10−7 (5) | 9.1 [7.3–18.2] × 10−6 (127) |
| shm2Δ | 1.2 [1.1–1.7] × 10−7 (2) | 1.7 [1.0–2.0] × 10−6 (23) | 5.5 [3.9–7.3] × 10−7 (8) | 3.6 [2.1–4.7] × 10−6 (50) |
| ura7Δ | 1.0 [0.9–1.5] × 10−7 (1) | 2.3 [1.3–4.1] × 10−5 (323) | 1.1 [0.7–1.5] × 10−6 (15) | 1.6 [1.1–2.6] × 10−5 (218) |
| rrm3Δ | 1.1 [0.8–1.5] × 10−7 (2) | 3.5 [2.1–4.4] × 10−7 (5) | 2.8 [1.9–4.8] × 10−6 (40) | 3.6 [2.6–6.0] × 10−6 (50) |
*
Median rates of inactivation of CAN1 gene (CanR) with 95% confidence interval in square brackets and fold increase relative to the WT in parentheses.