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. 2017 May 15;114(22):5683–5688. doi: 10.1073/pnas.1700861114

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Fig. 1. — XAF1 binds directly to MT2A. (A) Interaction of endogenously expressed XAF1 and MT2A. IB, immunoblot; WCL, whole-cell lysate. (B) In vitro pull-down assay showing a direct interaction between XAF1 and MT2A. (C) XAF1 constructs and its MT2A-binding status. (D) Identification of the ZF5 domain as a MT2A-binding region of XAF1. (E) XAF1 down-regulation of MT2A protein expression. (F) A crucial role for XAF1 ZF5 domain in binding and downregulating MT2A. (G) Immunofluorescence microscopic analysis showing no MT2A-reducing activity of ΔZF5-XAF1.