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. 2017 May 15;114(22):5683–5688. doi: 10.1073/pnas.1700861114

Fig. 2.

Fig. 2.

XAF1 promotes lysosomal degradation of MT2A. (A) Blockade of XAF1-induced MT2A reduction by Leupeptin. Cells were treated with tetracycline (1 μg/mL) and then exposed to MG132 (10 μM) or Leupeptin (10 μM) for 6 h. (B and C) A CHX chase assay showing XAF1-mediated MT2A destabilization. Cells were treated with CHX (40 μM) for the indicated hours. (D) Immunofluorescence analysis showing XAF1 stimulation of MT2A lysosomal translocation. (E) MT2A down-regulation of XAF1 protein expression. (F) Blockade of MT2A-induced XAF1 reduction by Leupeptin. (G and H) A CHX chase assay showing MT2A-mediated XAF1 destabilization (G) and comparison of XAF1 protein levels in control and MT2A-overexpressed cells following CHX treatment (H).