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. 2017 May 15;114(22):5683–5688. doi: 10.1073/pnas.1700861114

Fig. 6.

Fig. 6.

A XAF1-mediated free zinc increase drives a WT shift of p53 conformation. (A) A spectrofluorimetric measurement of Zinquin showing a XAF1 dose-associated increment of the intracellular free zinc level. (B) Up-regulation of WT p53 conformation and down-regulation of XIAP level by WT-XAF1 but not by ΔZF5-XAF1. PAb1620 and PAb240 antibodies were used to detect WT and mutant type (under nondenaturating gel conditions) of p53 conformation, respectively. Pan-p53 antibody was used to determine total p53 protein level. (C) MT2A inhibition of cytotoxic metal stress-induced p53 conformational change and XIAP reduction. (D) IP assay showing a role for XAF1 in genotoxic stress-induced p53 conformational change. (E) Opposite effects of XAF1 and MT2A on p53 conformation. (F) Comparison of XAF1 ability to induce apoptotic switch of ADR-treated HCT116 p53+/+ and p53−/− cells.