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. 2017 May 15;114(22):5683–5688. doi: 10.1073/pnas.1700861114

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Fig. S1. — Identification of MT2A and MT1A as a XAF1-binding protein. (A) Identification of MT2A as a XAF1-interacting protein in a yeast two-hybrid assay. (B) IP assay of the XAF1–MT2A interaction. IB, immunoblot; WCL, whole-cell lysate. (C) GST pull-down assay for the XAF1–MT2A interaction. (D) A dose-associated down-regulation of the MT2A protein level by ectopic expression of XAF1. (E) Induction of MT2A protein by siRNA-mediated depletion of XAF1 in HCC1937 cells. (F) GST pull-down assay for the XAF1-MT1A interaction. (G) A dose-associated down-regulation of the MT1A protein level by ectopic expression of XAF1. (H) Induction of MT1A protein by siRNA-mediated depletion of XAF1 in HCC1937 cells. (I) IP and IB assays showing a crucial role for the ZF5 domain of XAF1 in interaction with and down-regulation of MT1A.