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. 2017 May 15;114(22):5683–5688. doi: 10.1073/pnas.1700861114

Fig. S2.

Fig. S2.

MT2A and MT1A promote lysosomal degradation of XAF1. (A) Blockade of XAF1-induced MT1A down-regulation by Leupeptin. (B) Blockade of XAF1 inhibition of GFP-MT2A fluorescence by Leupeptin. (C) Immunofluorescence microscopic analysis showing XAF1-induced lysosomal translocation of MT1A. (D) MT1A down-regulation of XAF1 protein expression. (E) Blockade of MT1A-induced XAF1 down-regulation by Leupeptin. (F) MT2A down-regulation of WT-XAF1 but not of ΔZF5-XAF1. (G–I) Cotransfection assay showing mutual degradation of XAF1 and MT2A its blockage by Leupeptin.