Fig. S5.
Role for XAF1–MT2A antagonism in cellular response to genotoxic stress. (A) IB assay showing 5-FU–mediated XAF1 induction and effect of XAF1 depletion on MT2A expression. HCC1937-shControl and HCC1937-shXAF1 subline cells were treated with 5-FU (25 μM) for the indicated times. (B) Promotion of ET-induced apoptosis by WT-XAF1 but not by ΔZF5-XAF1. HCT116 cells transfected with increasing dose of either WT-XAF1 or ΔZF5-XAF1 were exposed to ET (50 μM) for 48 h. (C) Association of antiapoptotic function of EGF with its XAF1-downregulating effect in CdCl2-treated cells.