Fig. S7.

Hydrodynamic diameters of EHD2 in the closed and open conformation, related to Fig. 4. (A) Structure representation of EHD2 in closed and open conformation where the helical domains are tilted in a θ = 50° angle. The indicated distances illustrate the distance from residue L303 (labeled for FRET measurements as in Fig. 2C, main text) to the membrane interface in the two conformations. The horizontal line does not necessarily denote the actual position of the membrane after partial insertion of the lipid binding motif. The diameter is depicted in the side view for closed conformation and in the bottom view for the closed and open conformation. The distance measurements were acquired using the software PyMOL with PDB ID code 4CID as template. (B) Theoretical diameters as determined from the distances measured using PyMOL. (C) DLS autocorrelation curves of EHD2 and EHD2 ΔN in presence of ADP and table summarizing the hydrodynamic diameters deduced for EHD2 and EHD2 ΔN.