TABLE 2.
Transcript levels and promoter activities of selected AC2-inducible genes
Gene, promoter construct | Basal transcript level on control chips | Fold activation on AC2 chips | AC2 source | Relative CAT expression (%) in N. plumba protoplasts | Relative CAT expression (%) in A. thaliana protoplasts |
---|---|---|---|---|---|
CaMV 35S promoter | 100 | 100 | |||
MYMV | 76 | 99 | |||
ACMV | 70 | 95 | |||
At3g12460, hypothetical 3′-5′ exonuclease WEL-1 | 44 (17 to 76) | 0.9 | 0.6 | ||
43× | MYMV | 47 | 127 | ||
20× | ACMV | 4.3 | 7.1 | ||
At1g02813, expressed protein | 47 (27 to 62) | 1.1 | 0.4 | ||
57× | MYMV | 24 | 154 | ||
13× | ACMV | 5.8 | 28 | ||
At1g13610, hypothetical protein | 6 (3 to 9) | 0.2 | <0.01 | ||
56× | MYMV | 65 | 173 | ||
27× | ACMV | 25 | 36 | ||
At5g11540, putative FAD-like oxidase | 11 (4 to 20) | 18 | 4.3 | ||
72× | MYMV | 69 | 249 | ||
6× | ACMV | 79 | 109 | ||
At4g39675, expressed protein, similar to HIV-1 p17 | 37 (4 to 116) | 21 | 21 | ||
28× | MYMV | 83 | 363 | ||
39× | ACMV | 91 | 358 | ||
At5g15960, cold- and ABA-inducible protein KIN1 | 52 (32 to 84) | 14 | 14 | ||
23× | MYMV | 81 | 406 | ||
6× | ACMV | 102 | 175 |