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. 2017 May 15;114(22):5731–5736. doi: 10.1073/pnas.1700499114

Fig. 1.

Fig. 1.

Pharmacological and electrophysiological verification of YNT-185 as OX2R agonist in transfected cells and in native OX2R-expressing neurons in mouse brain slices. (A) The chemical structure of YNT-185. (B) YNT-185 potently stimulated intracellular Ca2+ accumulation in CHO/hOX2R cells in a dose–response manner (n = 12 for each curve). (C and D) Effects of suvorexant and EMPA on concentration response curve to YNT-185 (n = 12 for each curve). (E) Effect of YNT-185 on the firing rate of histaminergic neurons in TMN without TTX. YNT-185 (Upper Left) and orexin-A (OXA) (Upper Right) induced firing of histaminergic neurons. EMPA inhibited the YNT185-induced firing of histaminergic neurons (Lower). Typical examples (F) and quantitative data (G) about effect of YNT-185 on resting membrane potential of histaminergic neurons in TMN with TTX (n = 4 for each condition). (H) Histological identification of histaminergic neurons in TMN. A cell injected with neurobiotin through the patch pipet was immunoreactive to anti-HDC antibody. *P < 0.05.