TABLE 2.
Cellular gene expression as analyzed by Q-RTPCR compared to microarray analyses
| Gene | Change (n-fold)a | |||||||
|---|---|---|---|---|---|---|---|---|
| Q-RTPCR | Microarrayb | |||||||
| 12 h p.i. | 24 h p.i. | 32 h p.i. | 48 h p.i. | 12 h p.i. | 24 h p.i. | 32 h p.i. | 48 h p.i. | |
| β-Actin | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 
| G1P2 | 44 | −6.5 | −1.9 | −2.0 | 24.3 | 1.0 | 1.5 | 1.2 | 
| IFIT1 | 15 | 1.1 | 2.1 | 1.5 | 8.5 | NSDe | NSD | NSD | 
| CD83 | 1.05 | −2.2 | −1.5 | −1.1 | −1.7 | −3.2 | −1.2 | −1.6 | 
| JunB | n.a.d | −2.5 | −2.1 | −4.4 | 1.4 | −2.4 | −2.3 | −2.0 | 
| ATF2 | −1.1 | −0.9 | −1 | −1.8 | 1.0 | −1.2 | NSD | −1.2 | 
| MCCc | 1.2 | 1.6 | 2.6 | 1.6 | ||||
| IL-6 | 1.7 | −41 | −14.9 | −21.9 | −1.7 | −3.8 | NSD | −3.7 | 
| c-Myc | −1.4 | −2.5 | −1.6 | −2.8 | −1.8 | |||
| CTSF | n.a. | 1.4 | 2.8 | 3.3 | 1.1 | 1.2 | 2.6 | 2.2 | 
| ANPEP | n.a. | 0.8 | 1.4 | 1.5 | −1.1 | 1.1 | 1.9 | 1.8 | 
| CYR61 | 1.2 | −2.0 | −1.8 | −2.4 | NSD | −3.8 | −6.1 | −7.0 | 
| FUBP | n.a. | −1.0 | −0.6 | −0.8 | 1.0 | 1.0 | −1.5 | −1.3 | 
The factors in this table relate gene activities in Ad12-infected cells to those in mock-infected cells. All factors in this table were normalized relative to the activity of the β-actin gene, whose activity did not change in HeLa cells after Ad12 infection. Boldfaced entries indicate genes discussed in the text.
These data were taken from Table 1.
The MCC gene was not present on the DNA microarray.
n.a., not analyzed.
NSD, no signal detectable.