Fig. 4.
MgtS acts via MgtA. (A) The ability of MgtS overexpression to complement the ∆mgtS growth curve defect requires mgtA. The OD600 of cultures of ΔmgtS (GSO775) and ∆mgtS ∆mgtA (GSO781) carrying pBAD24 or pBAD-MgtS were measured at the indicated times as in Fig. 1C. The average of three cultures is shown. (B) Repression of Mg2+-reporter activity by MgtS is dependent on mgtA. β-Galactosidase activity was assayed for cultures of Plac-mgtA 5′-UTR-lacZ ΔmgtS (GSO774) or Plac-mgtA 5′-UTR-lacZ ΔmgtS ΔmgtA (GSO782) cells carrying pBAD24 or pBAD24-MgtS cells as described in SI Appendix. The average of three independent trials is shown, and the error bars represent one SD.