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Electron micrographs of CNGV harvested from infected KFC. The purified virus was negatively stained with 2% phosphotungstate (A and B). The particle size was in the range of 96 to 105 nm, with an average of 103 nm. Bar, 0.1 μm. Thin sections (C and D) were made for ultrastructural analysis by transmission electron microscopy. Purified virus pellets were fixed with 3% glutaraldehyde in 0.1 M sodium cacodylate and then stained with uranyl acetate and lead citrate.
