FIG. 4.

Identification of CNGV genome fragments by PCR (A and B) and Southern blotting (C). The AP1-AP2 (A) and AP1-AP3 (B) primers derived from clone A (Fig. 5) were used for PCR amplification. The templates for the PCR were as follows: total DNAs extracted from infected (TCin) and uninfected (TCun) KFC, CNGV DNA (CNGV), and DNAs from livers of sick and naïve fish (LI and LU, respectively) and from kidneys of sick and naïve fish (KI and KU, respectively). MW, HindIII-cleaved λ phage DNA. (C) A [α-³²P]dCTP-labeled probe derived from clone E (Fig. 5) was hybridized to a pBluescript II SK(−) plasmid containing viral fragment E (pCNGV), vaccine virus (VAC), HSV-1, CNGV, TCin, and TCun DNA samples. Labeled viral DNAs were detected with a phosphorimager.