Fig. 3. On-chip culture of GFP-expressing S. Typhimurium. Bacteria were first captured from 50 μL at 105 cfu ml–1 (5000 total bacteria perfused in the chamber), and then cultured on chip, perfusing LB at a flow rate of 0.15 μL min–1. (a) Images of the whole bed (lower frame) and zoom (upper frame), taken every 120 min. (b) Fluorescence intensity (green) and fluidized bed area (blue). (c) Towards the end of the bed expansion (300 min), a flow of bacteria can be seen leaving the magnetic beads, dragged by the flow (at this low resolution, bacteria flow appears as faint streaks).
