Table 6. RNAseq of four M. incognita life stages, sequencing, cleaning and mapping.
Sequencing | Cleaning | Mapping to the genome | ||||
---|---|---|---|---|---|---|
Libraries | Initial read pairs | Cleaned read pairs | Mapped read pairs | % mapped | % unique | % multi |
egg-1 | 87 074 210 | 48 295 627 | 46 074 870 | 95.40 | 76.08 | 19.32 |
egg-2 | 94 158 382 | 48 492 099 | 46 562 165 | 96.02 | 76.02 | 20 |
egg-3 | 66 074 037 | 32 259 963 | 30 478 818 | 94.48 | 75.17 | 19.3 |
J2-1 | 72 958 025 | 32 264 784 | 29 720 974 | 92.12 | 72.42 | 19.7 |
J2-2 | 78 380 200 | 36 426 756 | 33 827 380 | 92.86 | 72.17 | 20.69 |
J2-3 | 80 099 032 | 32 972 463 | 28 181 323 | 85.47 | 67.05 | 18.42 |
J3J4-1 | 92 349 648 | 18 410 414 | 16 356 975 | 88.85 | 69.75 | 19.1 |
J3J4-2 | 60 483 114 | 11 793 967 | 10 875 445 | 92.21 | 73.35 | 18.86 |
J3J4-3 | 84 491 183 | 14 725 039 | 13 804 525 | 93.75 | 72.52 | 21.23 |
adult-1 | 68 718 904 | 15 362 025 | 14 462 874 | 94.15 | 72.08 | 22.07 |
adult -2 | 76 861 805 | 16 904 124 | 13 226 056 | 78.24 | 59.74 | 18.5 |
adult -3 | 79 680 298 | 20 839 034 | 15 621 417 | 74.96 | 57.15 | 17.81 |