FIG. 6.

Structure of LuIII and LuΔ2 replication intermediates. Southern transfers of 2D agarose gels in which LuIII and LuΔ2 Hirt extracts harvested 22 h postinfection were subjected to electrophoresis first through a neutral agarose gel (as indicated by the horizontal arrow in panel A) in the presence of 0.2% SDS. An equivalent lane was turned through 90° and re-electrophoresed under denaturing conditions (in the direction indicate by the vertical arrow in panel A). Transfers were probed consecutively for positive- and negative-sense strands. DNA migrating as free single strands under native conditions is labeled s-s.