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. 2017 Apr 26;29(5):1157–1174. doi: 10.1105/tpc.16.00855

Figure 1.

Figure 1.

Interaction of Arabidopsis Flavonoids Biosynthetic Enzymes with KFB Proteins.

(A) Phylogeny of KFBs clustered with KFB01, 20, 39, and 50 (marked as bold). Scale shows the length of branch that represents an amount amino acid change of 0.5.

(B) Pairwise Y2H assays of the “bait” pDEST-GBKT7-CHS, -ANS or -F3′H with the “prey” pDEST-GADT7-At1g23390, -At1g30090, -At2g24540, -At3g61590, and -At4g03030. Yeast harboring the pairwise expression cassettes were grown on both the growth medium lacking Leu and Trp (SD-LT) and on the selective medium lacking Leu, Trp, His, and adenine (SD-LTHA) for 3 d.

(C) BiFC assays for the CHS-CFPc fusion with the truncated KFBCHS that had a deletion of its F-box motif and was fused with nYFP (F^KFBCHS-nYFP). The expression constructs were coinfiltrated into tobacco leaves according to the indicated combinations on the top of panels. The fluorescence distribution of CHS-full length of GFP fusion is shown in the left panel. Arrowheads indicate the nucleus. Bar = 200 μm.