Figure 7.

KFB^CHS Is Responsive to Different Qualities of Light.

Seedlings of the wild type (Col-0), kfb^chs mutant lines (kfb^chs -1, Salk_085384; kfb^chs -2, Salk_051263), and overexpression lines OE11 and OE14 were germinated as described in Methods and transferred to blue (36 µmol·m⁻²·s⁻¹), red (40 µmol·m⁻²·s⁻¹), or far-red (20 µmol·m⁻²·s⁻¹) light for 9 d at 22°C. Dark: the seedlings remained in the total darkness for 9 d.

(A) The accumulated levels of anthocyanin in the seedlings grown under different light conditions. Data represent means ± sd of three biological replicates. The seedlings harvested from each agar plate were regarded as one biological replicate. *P < 0.05 and **P < 0.01 (Student’s t test) compared with the corresponding control in the dark.

(B) Relative expression levels of KFB^CHS and CHS in the seedlings of the wild type, kfb^chs mutant, and overexpression lines under dark, or red, blue, and far-red light. Each gene’s expression level in the wild type in the dark was set as “1.” Data represent ± sd of three biological replicates as the defined in (A). For clarity, statistic indicators were only presented for KFB^CHS in the wild type. *P < 0.05 and **P < 0.01 (Student’s t test) compared with the sample in the dark.

(C) Immunoblot of CHS from wild-type seedlings under dark, or red, blue, and far-red light. Ten micrograms of total proteins for each sample were resolved on 12% SDS-PAGE gel and probed with anti-AtCHS antibody (upper). Total protein stained with Ponceau S serves as the loading controls (lower); note that the amount of Rubisco in the loading proteins also varied in the plants under different light conditions.

(D) Immunoblot of CHS from the seedlings of the wild type, kfb^chs mutant, and overexpression lines under different qualities of light. Total protein stained with Ponceau S serves as loading controls.