FIG. 2.

Indirect IFAs after transfection of mutant WN virus RNAs. RNAs were derived by transfection of wt and mutant WN virus genome-length DNAs and used to transfect hamster kidney cells (BHK-21). On the days indicated, cells were replated on a chamber slide, and IFA was performed by standard methods with a polyclonal mouse anti-WN virus hyperimmune ascitic fluid on the days indicated. The nucleotide sequences of the 3′SLs in mutant C1, A1L, A3, E, and F1 RNAs are shown in Fig. 3A, 5, 6, and 8.