FIG. 7.

Nucleotide sequences of the long stem and loop of 3′SLs present in A61C, G20U, and 2U RNAs are depicted. A horizontal line indicates the boundary between the top (t) and bottom (b) portions of the wt WN virus 3′SL (3, 4, 42). The TEF-binding domain in all three mutant nucleotide sequences is indicated by brackets and an asterisk to the left. For the A61C and G20U mutations, the loci of the substituted nucleotides are indicated in large, boldface type. For the 2U 3′SL, the U residues replacing A61 and G20, respectively, are shown in large boldface type. In the G20U nucleotide sequence, nucleotide A60, which spontaneously reverted to C in a portion of RNAs after transfection, is circled.