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. 2015 May 5;7:30–37. doi: 10.1016/j.btre.2015.04.008

Fig. 1.

Fig. 1

Characterization of wild-type AvHaCE. (A) Coomassie blue stained SDS-PAGE gel (12.5%) of purified AvHaCE. Lane 1, molecular mass standards; lane 2, AvHaCE after purification by immobilized metal affinity chromatography with Ni–NTA as the matrix. (B) HPLC analyses of wild-type AvHaCE reaction product and guanine nucleotide standards, as indicated. (C) MALDI analysis of the HPLC purified AvHaCE reaction product. The species with a molecular mass of 558 corresponds to pGp. (D) Time-course for pGpG production from GTP with wild-type AvHaCE. To correct for variations in injection volumes, pGpG and GTP peak areas were normalized to peak areas of a co-injected NAD standard.