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. 2015 May 5;7:30–37. doi: 10.1016/j.btre.2015.04.008

Fig. 6.

Fig. 6

HPLC analysis of radiolabeled cyclic-diGMP synthesis by the AvHaCE AAL variant. Both radioactivity and UV–vis absorbance were monitored, as indicated. A slight shift in the detection of radioactivity and absorbance occurs because of the delay that occurs as eluted material flows first through one detector then the other. (A) Reaction products after 15 min incubation at room temperature. (B) Reaction products after 60 min incubation at room temperature.