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Ct and RI values of influenza virus in the RT-qPCR SYBR Green method.
The standard curve of the threshold cycle (Ct) and relative intensity values (RI) of type A influenza (panels A, B) and type B influenza (panels C, D). The culture supernatant of influenza virus (type A: A/Okuda/57, type B: B/Osaka/304/2007) was diluted to the 0.5, 1, 2, 4, 7, 10, 100, 1000, and 10,000 copies per sample. Three independent experiments were performed in triplicate.
