Fig. 4.

Analysis of monomeric and dimeric G4 folding of relevant oligonucleotides. (A) Native gel electrophoresis at increasing oligonucleotide concentration (20, 45, 120, 415, 1600, 6200 nM). Samples identity is indicated above the gel image. Marker oligonucleotides with known length and unable to fold into secondary structures are loaded. (B) CD spectra of G4s showing dimeric folding and their corresponding monomolecular form when one T base was added at each of the two ends. (C) EMSA analysis of NCL binding. Oligonucleotides identity is shown above each lane. (D) Quantification of NCL/G4 complex bands obtained in the EMSA in panel (C).