Fig. 5.

Analysis of NCL binding towards oligonucleotides with variable G4s conformation. (A) CD spectra of 313, 333, 363 oligonucleotides folded in the presence of 20% (w/v) PEG200 and K⁺ or Na⁺. (B) EMSA analysis of NCL binding to 313, 333, 363 oligonucleotides folded in the presence of 20% (w/v) PEG200 and K⁺ or Na⁺ as indicated above the gel image. (C) Quantification of NCL/G4 complex bands obtained in the EMSA in panel (B). For the K⁺ or Na⁺ experiment series, binding was normalized to the intensity of the 333 NCL/G4 complex band in the corresponding salt condition.