Table 2.
Stability (Tm values) of oligonucleotides of the (GGGTTT)5 series with G-tracts replaced with Ts. Tm were measured by CD thermal unfolding and reported at the positive CD peaks, signatures of the parallel (265 nm) and antiparallel (285 nm) G4 conformation contribution.
| Oligonucleotide | Tm (265 nm) (°C) | Tm (285 nm) (°C) |
|---|---|---|
| (GGGTTT)4 | 66.6 ± 0.4 | 66.1 ± 0.4 |
| (GGGTTT)5 | 65.5 ± 1.1 | 64.5 ± 0.5 |
| n5M1 | 58.0 ± 0.8 | 55.7 ± 0.5 |
| n5M2 | 53.4 ± 0.5 | 54.5 ± 0.2 |
| n5M3 | 56.7 ± 0.3 | 56.6 ± 0.1 |
| n5M4 | 52.0 ± 0.3 | 53.2 ± 0.1 |
| n5M5 | / | 60.4 ± 0.4 |