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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Invest Dermatol. 2016 Oct 26;137(3):576–586. doi: 10.1016/j.jid.2016.09.033

Figure 4. JP4-039 applied to human skin demonstrates potent antioxidant function and prevents skin damage from radiation induced oxidative stress.

Figure 4

60 Gy Irradiated human skin was treated topically with JP4-039 or not 30 min after irradiation and harvested 24h later for analysis. Depicted are H&E stained sections (bar = 40μm) (a), apoptosis as determined by TUNEL (b), percent positive TUNEL cells (d), and Caspase 3/7 levels (e). GSH levels were determined enzymatically (f). DNA damage was determined by 8-oxoguanine antibody (inset bar = 10μm) (c), and percentage of positive cells quantitated (g). Carbonyl protein levels were determined by ELISA (h). Data were analyzed as described in Figure 3.