Table 1. Recipe for sucrose density gradient preparation.

	10% sucrose solution (for 8 ml)	50% sucrose solution (for 8 ml)
Sucrose	0.8 g	4.0 g
2 M KCl	400 µl	400 µl
1 M MgCl2	40 µl	40 µl
100 mM DTT	160 µl	160 µl
1 M HEPES pH 7.4	160 µl	160 µl
100 mg/ml Cycloheximide	8 µl	8 µl
DEPC water	Upto 8 ml	Upto 8 ml