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. 2014 Dec 13;5:77–88. doi: 10.1016/j.btre.2014.12.004

Table 1.

SSF processes reported in literature.

Biocatalysts Fermentation conditions Ethanol concentration Yielda Reference
Simultaneous saccharification and fermentation (SSF)
(a mixture of various enzymes)
Inulinase + Z. moblilis 35 °C, continuous 55.1 g/L/h 95% Kim [128] 1990
A. niger 817 + S. cerevisiae 1200 30 °C, 15 h 10.4% (v/v) 92% Nakamura et al. [130]
1996



K. fragilis + 
Z. moblilis 3881
30 °C, 72 h 9.9% (v/v) 94% Szambelan et al. [131]
2005
K. fragilis + 
S. cerevisiae Bc16a
30 °C, 72 h 9.1% (v/v) 86%



A. niger SL-09 + 
S. cerevisiae Z-06
30 °C, 48 h 19.6% (v/v) 90% Ge and Zhang [132] 2005



Simultaneous saccharification and fermentation (SSF)
(yeast with inulinase activity)
K. fragilis 28 °C, 6 days 11.1% (v/v) 98% Guiraud et al. [133]
1981
K. marxianus 28 °C, 6 days 11.5% (v/v) 98%



K. marxianus ATCC12708 28 °C, 30 h 14 g/L 87% Duvnjak et al. [134]
1981
K. marxianus ATCC 10606 28 °C, 30 h 12 g/L 83%
K. cicerisporus ATCC 22295 28 °C, 30 h 13 g/L 86%
K. fragilis 105 28 °C, 30 h 11 g/L 79%



K. marxianus UCD 55-82 35 °C, 60 h 44 g/L 88% Bajpai and Margaritis [138],
K. marxianus UCD 55-82 35 °C, continuous 7 g/L/h 90% Sachs et al. [106],
1982



K. marxianus CECT 10875 28 °C, 30 h 19 g/L 96% Negro [150], 2006
K. cicerisporus Y179 30 °C, 144 h 12.3%(v/v) 86.9 Yu et al. [152], 2010
S. cerevisiae KCCM50549 30 °C, 36 h 32.6 g/L 70% Lim et al. [151] 2011



K. marxianus ATCC8554 35 °C, 84 h 60.9 g/L 87% Yuan et al. [153], 2008
S. cerevisiae 6525 with cloned inulinase gene 35 °C, 48 h, 72.5 g/L 85% Yuan et al. [155], 2013
K. marxianus with overexpressed inulinase gene 35 °C, 72 h, 96.2 g/L 93% Yuan et al. [156] 2013



Saccharomyces sp W0 with exo-inulinase gene 28 °C, 144 h 12.5%(v/v) 62.5% Zhang et al. [157] 2010
Saccharomyces sp W0 with 18S rDNA integration of exo-inulinase gene 28 °C,120 h 12.6%(v/v) 66% Yuan et al. [156] 2011
Saccharomyces sp W0 with endo-inulinase gene 30 °C, 120 h 12.6%(v/v) 65% Li et al. [159] 2013



K. marxianus PT-1 40 °C, 84 h 73.6 g/L 90% Hu et al. [160] 2012
or S. cerevisiae JZ1C 40 °C, 84 h 65.2 g/L 79.7%
S. cerevisiae DQ1 30 °C, 72 h 128.1 g/L 73.5% Guo et al. [161] 2013
a

Ethanol yield is the percentage of theoretical yield on the basis of total sugar in feedstock.