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. 2014 Dec 18;5:89–97. doi: 10.1016/j.btre.2014.12.005

Fig. 4.

Fig. 4

Physical cell disruption methods (a) freeze and thaw along with osmotic shock, (b) osmotic shock at differen temperatures. Optimization of osmotic shock for (c) time of application and (d) A. halophila cell density.