Fig. 2.

(a) Chromatographic and (b) spectral identification of the metabolite phenylacetic acid during styrene degradation of strains Kp5.2, CWB2, and 1CP.

Cell suspensions of Sphingopyxis sp. Kp5.2, Gordonia sp. CWB2, and Rhodococcus opacus 1CP were cultured and induced as described in the Section 2 and subsequently incubated for 12 h in the presence of 25 μmol gaseous styrene in 500-ml flasks. Culture supernatant was subjected to HPLC under conditions described in Section 2.