Fig. 5.

Applicability of strain ST for the formation of 4-chloro- and 4-chloro-α-methylphenylacetic acid.

Pseudomonas fluorescens ST (0.41 mg_cdw ml⁻¹) was incubated for 348 days in the presence of in total 3630 μmol 4-chlorostyrene (added in 21–42-μmol portions) (a) or for 25 days with overall 374 μmol 4-chloro-α-methylstyrene (added in 20–41-μmol portions) (b) as described in Section 2. Product formation was quantified by HPLC (see Section 2). Mean values and standard errors of four independent measurements are given.